Calcium Signaling and Patch Clamp

 

CALCIUM SIGNAL COMMITTEE PROGRAM

Lecture 1 Preparation of solution (1xPBS, calcium buffer, Calcium Free Buffer etc.) Theoretical
Lecture 2 Calcium Signaling Analysis-1 Practical
Lecture 3 Calcium Signaling Analysis-1 Practical
Lecture 4 Caspase 3, Caspase 9 and Apoptosis Analysis Practical
Lecture 5 Analysis of intracellular ROS and mitochondrial depolarization Practical
Lecture 6 Participants Making the Calcium Signaling Analysis-1 Practical
Lecture 7 Participants Making the Calcium Signaling Analysis-2 Practical
Lecture 8 Participants apoptosis, caspase-3, -9, Making the intracellular ROS and mitochondrial depolarization analysis. Practical
Lecture 9 Calculation of results, statistical analysis, and ready to be presented in the article-Making Drawing Figures Practical
Lecture10 Group discussions and advice Theoretical

Patch Clamp Course Program

Lecture 1

What is Patch Clamp? Introduction to Patch Clamp technique

Theoretical

Lecture 2

Preparing intra and extracellular solutions and ion channel specific agonists and antagonists

Practical

Lecture 3

Fabricating pipettes and initiating Patch Clamp recordings

Practical

Lecture 4

Patch Clamp recordings from DRG neurons

Practical

Lecture 5

Patch Clamp recordings from DRG neurons

Practical

Lecture 6

Patch Clamp recordings from DRG neurons

Practical

Lecture 7

Preparing secondary cell cultures and Patch Clamp recordings from Neuroblastoma cells

Practical

Lecture 8

Patch Clamp recordings from cell lines

Practical

Lecture 9

Analysis of recordings and form to publishable format

Practical

Lecture10

Group discussions and advice

Theoretical