Western Blot & RT-PCR

Western Blot & RT-PCR

Lecture 1

İntroduction to Western Blotting

  • Principles

  • Data Analysis

  • Troubleshooting

Theoretical

Lecture 2

Introduction to WB equipment

Practical

Lecture 3

Sample and Gel preperation

Practical

Lecture 4

Loading samples and running the gel

(Electrophoresis)

Practical

Lecture 5

Electro Transfer (Transfer of proteins to membrane)

Practical

Lecture 6

Blocking the membrane and Incubation with the primary antibody

Practical

Lecture 7

Incubation with Secondary Antibody

Practical

Lecture 8

Imaging ( Dark room applications ve Imaging System app.)

Practical

Lecture 9

Interpretation of results

Theoretical + Practical

Lecture 10

Group discussions and advice

Theoretical